TransStart® FastPfu Fly DNA Polymerase is a hot start, ultra high-fidelity DNA Polymerase used for fast PCR. Compared withTransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate (≤ 5 kb fragments can achieve 12 kb/min extreme amplification, > 5 kb fragments can achieve 6 kb/min high-speed amplification), featuring higher amplification efficiency, higher yield, higher fidelity and higher specificity. 2×TransStart® FastPfu Fly Reaction Mix already contains dNTPs. When DNA is amplified, just add template, primer, water and TransStart® FastPfu Fly DNA Polymerase. The Reaction can be carried out with the concentration of Reaction Mix being 1×. PCR products are blunt end and can be cloned directly into pEASY®-Blunt series of vectors.
• Offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• PCR products can be directly cloned into pEASY ®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• Complex templates, GC/AT-rich templates.
• Ultra high-fidelity and fast PCR, blunt end cloning, site-directed mutagenesis.
• Long fragment amplification.
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
