Product Catalog

Fast MultiSite Mutagenesis System

Catalog Number: FM201-01

Price:Please inquire first

Specification:
10 rxns
Quantity:
-
+
Manual MSDS
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Product Details

Fast MultiSite Mutagenesis System is used for generating mutated PCR fragments by introducing mutation sites on overlapping regions. High fidelity TransStart® FastPfu PCR SuperMix is included for amplification. This kit uses proprietary assembly mix and homologous recombination to seamlessly assemble up to six mutagenesis fragments.

• Fast: Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination.
• Flexible: Able to be cloned into any site to realize single-site/ multi-site, continuous/non-continuous mutagenesis.
• Efficient: >90% mutagenesis efficiency.

Notes
We suggest performing 25 cycles for PCR. For low yield PCR products, we suggest using up to 30 cycles.

Storage
at -20°C for two years

Shipping
Dry ice (-70℃)

Product Composition


ComponentFM201-01 (10 rxns)
TransStart® FastPfu Fly PCR SuperMix1 ml
DMT Enzyme (10 units/μl)30 μl
2×Assembly Mix50 μl
DMT Chemically Competent Cell10×50 μl
Nuclease-free Water1 ml


References

1 Li L, Fang C, Zhuang N, et al. Structural basis for transcription initiation by bacterial ECF σ factors[J]. Nature communications, 2019.(IF 11.90)

2 Deng L, Ren R, Liu Z, et al. Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis[J]. Nature communications, 2019.(IF 10.70)

3 Wang Z, Wang S, Xu Z, et al. Highly promiscuous flavonoid 3-O-glycosyltransferase from Scutellaria baicalensis[J]. Organic letters, 2019.(IF 6.55)

4 Zhang L, Jiang D, Li Y, et al. Characterization of a novel type III alcohol dehydrogenase from Thermococcus barophilus Ch5[J]. International Journal of Biological Macromolecules, 2021.(IF 6.95)

5 Zhang L, Wang L, Wu L, et al. Biochemical characterization and mutational studies of a thermostable endonuclease III from Sulfolobus islandicus REY15A[J]. International Journal of Biological Macromolecules, 2021.(IF 6.95)


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