Low temperature Uracil-DNA Glycosylase (UDG) is a recombinant protein derived from psychrophile marine bacteria that have been modified to be expressed in Escherichia coli. UDG enzyme can effectively hydrolyze the N-glycosidic bond between the uracil (dU) base and deoxyribose in single or double-stranded DNA, thus releasing free uracil and producing pyrimidine deficiency sites, which are mainly used to eliminate the aerosol contamination caused by PCR products containing dU. This product is easily hydrolyzed and fractured at high temperatures or pH. Compared with conventional UDG enzymes, the product  can operate at a lower temperature (20°C), i.e., when preparing qPCR or qRT-PCR reaction systems at room temperature, it can degrade contaminated dU-containing templates. This enzyme does not degrade dU-containing cDNA (DNA/RNA hybrid form) produced during reverse transcription. This product has high activity in most PCR reaction buffers and is suitable for PCR/qPCR, RT-PCR/qRT-PCR, and LAMP/RT-LAMP.

Applications 

Remove uracil base from single-stranded or double-stranded DNA; prevent aerosol contamination of PCR products containing dU and improve the specificity of the reaction.

Storage

at -20°C  for two years

Shipping

Dry ice (-70 ℃)