This product catalyzes the conversion of ATP into AMP and incorporates into the 3´ end of RNA sequentially, which doesn’t rely  on the presence of templates, that is, adding Poly(A) to the 3´end of mRNA. Polyadenylation modification increases the stability  of RNA in cells and enhances the efficiency of RNA expression after transfection or microinjection. This product has a very high  tailing efficiency, and can be used in the modification of mRNA tailing after in vitro transcription (JT101) and capping (LC101),  preparing for RNA samples used for cell transfection. 

Applications 

1.Adding Poly(A) tail to the 3’ end of mRNA after in vitro transcription (T7 High Efficiency Transcription Kit, Cat.No. JT101)  and capping (mRNA Capping Kit, Cat.No. LC101), which can stabilize the structure of 5’ cap and enhance the stability and  translation efficiency of mRNA in eukaryotic cells. 

2. Adding Poly(A) tail for cDNA synthesis, providing binding sites for oligo-dT primer. 

3. Label the 3´ ends of RNA by modified ATP or its analogs.