This product is designed for the quantitative detection of HEK293 cell DNA in various biological products by probe-based qPCR.  It can be used together with magnetic bead-based host cell residual DNA extraction kit (Cat. No.: EH201). The 2×HEK293 qPCR  SuperMix included in this product contains PerfectStart Taq hot start enzyme (which utilizes three monoclonal antibodies to  efficiently bind with Taq DNA polymerase, effectively blocking the enzyme's activity and preventing non-specific amplification at  low temperature), a specially optimized qPCR reaction buffer for HEK293 cell DNA detection, dNTPs, PCR enhancer, and stabilizer.  In addition, a dUTP/UDG system is introduced into this reaction mix, which can degrade U-containing ssDNA and dsDNA before  reverse transcription, eliminating carry-over contamination caused by PCR products.  

· Three-antibody blocking technology ensures high specificity, high sensitivity, high amplification efficiency, and broad sample  compatibility.

· Specially optimized qPCR reaction buffer provides faster extension speed, higher sensitivity and specificity.

· Incorporates UDG enzyme and dUTP to effectively prevent carry-over contamination from PCR products, ensuring accurate data.

at -20°C for two years

Dry ice (-70℃)