Product Catalog

pEASY®-Blunt El Expression Kit

Catalog Number: CE111-01

Price:Please inquire first

Specification:
10 rxns

Product Details

pEASY®-Blunt El Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa. 


•5 minutes fast ligation of P/u-amplified PCR products.

• Ampicillin resistance.

•T7 promoter primer and T7 terminator primer for sequencing.

•Bacteriophage T7 lac promoter for high level expression.

•N-terminal 6xHis tag for easy purification.

•Transl-Tl phage resistant chemically competent cell, high transformation efficiency(> 109 cfu/µg pUC19 DNA) and fast growmg.

•El Expression Plasmid included as negative control.


Storage 

Trans 1-Tl Phage Resistant Chemically Competent Cell at -70℃ for six months; others at -20℃ for nine months 


Shipping

Dry ice (-70℃)

Product Composition

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References

Yin Y R , Hu Q W , Xian W D , et al. Characterization of a neutral recombinant xylanase from Thermoactinospora rubra YIM 77501T[J]. 2017,110(3):429-436.

Yang N , Zhang D F , Tao Z , et al. Identification of a novel class B scavenger receptor homologue in Portunus trituberculatus: Molecular cloning and microbial ligand binding[J]. Fish & Shellfish Immunology, 2016, 58:73-81.

Liang M , Yang W , Su S , et al. Genome-wide identification and functional analysis of S-RNase involved in the self-incompatibility of citrus[J]. Molecular Genetics and Genomics, 2017, 292(2):325-341.

Ju Z , Cao D , Gao C , et al. A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants[J]. Plant Physiology, 2017, 173(4):1940-1952.

Li L , Zheng M , Long H , et al. Molecular Cloning and Characterization of Two Genes Encoding Tryptophan Decarboxylase from Aegilops variabilis with Resistance to the Cereal Cyst Nematode (Heterodera avenae) and Root-Knot Nematode (Meloidogyne naasi)[J]. Plant Molecular Biology Reporter, 2016:1-10.

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