Human  IL-12 p70 ELISA Kit

Interleukin-12 (IL-12), also known as IL-12 p70, is a heterodimer composed of two subunits IL-12A (p35) and  IL-12B (p40) , with a molecular weight of 70-75 kD. IL-12 p70 is a secreted protein that is mainly produced and  secreted by macrophages, dendritic cells, monocytes, neutrophils or other antigen-presenting cells in response to  antigenic stimulation, it plays an important role in the maturation, activation, proliferation and immune regulation of  immune cells. As a T cell stimulator, IL-12 p70 can effectively promote the proliferation and function of T cells, and  participate in the differentiation of naïve T cells into Th1 cells. IL-12 p70 is a cytotoxic lymphocyte maturation factor.  In the tumor microenvironment, IL-12 p70 stimulates activated T cells and natural killer cells to release toxic enzymes  or secrete effector cytokines such as IFN-γ. These effector molecules are critical for tumor clearance. This kit uses Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) to measure the amount of IL-12 p70 in  human serum and plasma. The ELISA microplate in the kit is precoated with a high-affinity anti-human IL-12 p70  antibody. The standard or test sample is added to the microplate wells. After incubation, IL-12 p70 present in the  sample will be bound specifically to the precoated antibody on the microplate wells. After washing, biotin-labeled  anti-human IL-12 p70 detection antibody is added to the microplate wells. After re-incubation, the detection antibody  will be bound specifically to IL-12 p70 anchored on the microplate wells. Subsequently, the horseradish  peroxidase-labeled streptavidin (Streptavidin-HRP) is added to the microplate wells and incubated. Biotin and  streptavidin on the detection antibody produces the "coated antibody-human IL-12 p70 protein-detection  antibody-Streptavidin-HRP" immune complex through high-strength non-covalent binding. After washing again, the  chromogenic substrate TMB is added to the wells. HRP catalyzes the TMB substrate to create blue coloration, with  intensity positively correlated with the concentration of IL-12 p70 in the sample. The reaction is stopped by adding  stop solution, and the absorbance is measured at 450 nm (reference wavelength 570-630 nm). A standard curve is  drawn to calculate the concentration of IL-12 p70 in the sample based on the absorbance value. This kit is highly  specific, has high detection sensitivity and is convenient to use.

Suitable Sample Types 

Serum, plasma, etc.

Storage

at 2~8°C in the dark for one year.

Shipping
Ice bag(4°C)