The kit is designed to prepare CUT&Tag libraries for mammalian cells for the Illumina high-throughput sequencing platform. The prepared libraries can be used for Single-end sequencing and Paired-end sequencing. Cleavage Under Target & Tagmentation (CUT&Tag) is a new method for studying protein interactions with DNA. Protein A/G-Tn5 transposase fusion protein and the target protein can form an immune complex through antibody bridging. Tn5 transposase is activated in the presence of Mg2+, and so short sequences containing adapters can be inserted into both ends of the DNA fragment that binds to the target protein. Finally, library can be constructed by PCR amplification. The kit is suitable for DNA research on the interaction of DNA-binding proteins such as transcription factors and co-regulators. Compared with traditional ChIP-seq, it has the advantages of more accurate and stable protein-DNA interaction information in low input of cells.
Applications
102-105 mammalian cells, or processed cell nucleus, and eukaryotic cells without cell wall (such as plant cell protoplasts, etc.).
Storage
Box 1 is stored at 4°C for 1 year and Box 2 is stored at -20°C for 1 year.
Shipping
Dry ice (-70℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
