Interleukin-12 (IL-12), also known as IL-12 p70, is a heterodimer composed of two subunits IL-12A (p35) and IL-12B (p40) , with a molecular weight of 70-75 kD. IL-12 p70 is a secreted protein that is mainly produced and secreted by macrophages, dendritic cells, monocytes, neutrophils or other antigen-presenting cells in response to antigenic stimulation, it plays an important role in the maturation, activation, proliferation and immune regulation of immune cells. As a T cell stimulator, IL-12 p70 can effectively promote the proliferation and function of T cells, and participate in the differentiation of naïve T cells into Th1 cells. IL-12 p70 is a cytotoxic lymphocyte maturation factor. In the tumor microenvironment, IL-12 p70 stimulates activated T cells and natural killer cells to release toxic enzymes or secrete effector cytokines such as IFN-γ. These effector molecules are critical for tumor clearance.

This kit uses Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) to measure the amount of IL-12 p70 in human serum and plasma. The ELISA microplate in the kit is precoated with a high-affinity anti-human IL-12 p70 antibody. The standard or test sample is added to the microplate wells. After incubation, IL-12 p70 present in the sample will be bound specifically to the precoated antibody on the microplate wells. After washing, biotin-labeled anti-human IL-12 p70 detection antibody is added to the microplate wells. After re-incubation, the detection antibody will be bound specifically to IL-12 p70 anchored on the microplate wells. Subsequently, the horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is added to the microplate wells and incubated. Biotin and streptavidin on the detection antibody produces the "coated antibody-human IL-12 p70 protein-detection antibody-Streptavidin-HRP" immune complex through high-strength non-covalent binding. After washing again, the chromogenic substrate TMB is added to the wells. HRP catalyzes the TMB substrate to create blue coloration, with intensity positively correlated with the concentration of IL-12 p70 in the sample. The reaction is stopped by adding stop solution, and the absorbance is measured at 450 nm (reference wavelength 570-630 nm). A standard curve is drawn to calculate the concentration of IL-12 p70 in the sample based on the absorbance value. This kit is highly specific, has high detection sensitivity and is convenient to use.

at 2~8°C in the dark for one year.

ice bag (4℃)