pEASY®-Uni Seamless Cloning and Assembly Kit
Catalog Number： CU101-01
Price：Please inquire first
Product Details
This kit takes advantage of proprietary recombinases and homologous recombination to achieve directional recombination of PCR inserts with 15-25bp overlapping ends into any linearized vector, enabling efficient and seamless assembly of 1-7 fragments.
• Fast: 5~15 minutes
• Simple: No restriction enzyme digestions.
• High efficiency: > 95% cloning efficiency.
• Seamless: No extra sequences introduced.
Single or multi fragments cloning and assembly.
Trans1-T1 Phage Resistant Chemically Competent Cells at -70℃ for six months; others at -18℃ or below for two years
Dry ice (-70℃)
| Component | CU101-01 | CU101-02 | CU101-03 |
| 2×Assembly Mix | 50 μl | 100 μl | 3×100 μl |
| Linearized pUC19 Control Vector (10 ng/μl) | 3 μl | 6 μl | 18 μl |
| Control Insert (1 kb，20 ng/μl） | 3 μl | 6 μl | 18 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cell | 5×100 μl | 10×100 μl | 30×100 μl |
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