pEASY®-T3 Cloning Kit
Catalog Number： CT301-01
Price：Please inquire first
Product Details
pEASY®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released from a single enzyme digestion.
•5 minutes fast ligation of Taq-amplified PCR products.
•Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter and SP6 promoter for in vitro transcription.
•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Competent Cell at -70°C for six months; others at -20°C for nine months
Shipping
Dry ice (-70℃)
| Component | CT301-01 (20 rxns) | CT301-02 (60 rxns) |
| pEASY ®--T3 Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
1 Jia H, Liu Y, Li X, et al. Windborne migration amplifies insect-mediated pollination services[J]. Elife, 2022.(IF 8.14)
2 Liu F, Li X, Zhao M, et al. Ultrabithorax is a key regulator for the dimorphism of wings, a main cause for the outbreak of planthoppers in rice[J]. National Science Review, 2020.(IF 16.69)
