pEASY®-T1 Simple Cloning Kit
Catalog Number： CT111-01
Price：Please inquire first
Product Details
pEASY®- T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products.
•5 minutes fast ligation of Taq-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•SR primer and M13 forward primer for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months
Shipping
Dry ice (-70℃)
| Component | CT111-01 (20 rxns) | CT111-02 (60 rxns) |
| pEASY ®-T1 Simple Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| SR Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
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