Trans1-Blue Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA). The competent cell is resistant to tetracycline (TetR).
Genotype
recA1 endA1 gyrA96 thi-1 hsdR17 supE44 (rk-,mk+), relA1 lac [F' proAB lacIqZΔM15: Tn10 (TetR)]
Features
• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
• TetR.
• Blue/white selection.
Storage
at -70°C for six months. Do not store in liquid nitrogen
Shipping
Dry ice (-70℃)
Xue X , Wang R , Tu T , et al. The N-Terminal GH10 Domain of a Multimodular Protein from Caldicellulosiruptor bescii Is a Versatile Xylanase/β-Glucanase That Can Degrade Crystalline Cellulose[J]. Applied and Environmental Microbiology, 2015, 81(11):3823-3833.
Li D , Lv L , Chen J C , et al. Controlling microbial PHB synthesis via CRISPRi[J]. Applied Microbiology and Biotechnology, 2017, 101(14):5861-5867.
3.Yuru T , Ping S , Yujun Z , et al. Molecular Cloning and Characterization of DXS and DXR Genes in the Terpenoid Biosynthetic Pathway of Tripterygium wilfordii[J]. International Journal of Molecular Sciences, 2015, 16(10):25516-25535.
Zheng Y , Yuan Q , Yang X , et al. Engineering Escherichia coli, for poly-(3-hydroxybutyrate) production guided by genome-scale metabolic network analysis[J]. Enzyme & Microbial Technology, 2017, 106:60.
Li L , Yang J , Li J , et al. Role of two amino acid residues' insertion on thermal stability of thermophilic a-amylase AMY121 from a deep sea bacterium Bacillus sp. SCSIO 15121[J]. Bioprocess and Biosystems Engineering, 2015, 38(5):871-879.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
