Mycoplasma is a prokaryotic organism which should be tested routinely for all cell stocks and all new cultures entering a laboratory due to its threat to cell physiology and experiment results and its widespread in cell culture. TransGen Biotech provides a full set of products related to mycoplasma, including 2 detection kits based on luciferase and PCR, elimination and prevention reagents. In the past few years, these products have been received recognition and positive feedbacks from our customers. To reward our customers’ support and assist more customers with their cell culture, we decided to launch the promotion event of Buy 5 & Get 1 Free. Choose one for free from any of the following products for a single order purchasing 5 products.
Don’t miss this chance to stock up!
TransDetect® Luciferase Mycoplasma Detection Kit（FM301）
This product is designed using an enzyme unique to mycoplasma. This enzyme degrades MycoDetect Substrate and converts ADP to ATP. Luciferase catalyzes oxidation of luciferin to produce bioluminescence in the presence of ATP. Cell cultures can be characterized for the presence of mycoplasma contamination by detecting bioluminescence.
Features
• Fast: only 20-30 minutes.
• More accurate results: only detect bioactive mycoplasma.
• Wide range of applications: detection of a wider range of types.
TransDetect® PCR Mycoplasma Detection Kit（FM311）
This product detects the presence of mycoplasma contamination in biological materials such as cultured cells by PCR method. Highly specific primers have been designed to amplify a fragment of 16S rRNA coding DNA that is conserved across all commonly-known mycoplasma species. The kit includes an optimized supermix and primer, ultrapure water and positive control template. Using this kit, cell culture supernatants can be tested directly without DNA extraction.
Features
• High sensitivity: able to detect as low as 20 copies of mycoplasma genome.
• High specificity: only detect mycoplasma DNA, not eukaryotic or bacterial DNA.
• Easy to use: no need to extract genomic DNA, suitable for the detection of a large number of cell samples.
• Positive control and negative control to ensure the accuracy of PCR test results.
Mycoplasma subtypes that can be detected accurately by FM311 and FM301
This includes, but is not limited to, the following subtypes:
M. Alkalescens, M. canadense, M. arginini, M. hominis，M. bovoculi, M. arthritidis, M. neophronis, M. salivarium, M.ovipneumoniae, M. zalophi, M. orale, M. hyorhinis, M. pneumoniae, M. hyosynoviae
Many products were cited in prestigious journals (Selected Citations)
FM301
Bak instead of Bax plays a key role in metformin-induced apoptosis s in HCT116 cells (Selected Citations)
https://www.nature.com/articles/s41420-021-00755-y
FM311
Modulating TRADD to restore cellular homeostasis and inhibit apoptosis (Selected Citations) https://doi.org/10.1038/s41586-020-2757-z
Mitochondrial base editor induces substantial nuclear off-target mutation (Selected Citations) https://www.nature.com/articles/s41586-022-04836-5
Rapamycin Pretreatment Rescues the Bone Marrow AML Cell Elimination Capacity of CAR-T Cells https://aacrjournals.org/clincancerres/article/27/21/6026/671738/Rapamycin-Pretreatment-Rescues-the-Bone-Marrow-AML
miR-192-5p Silencing by Genetic Aberrations Is a Key Event in Hepatocellular Carcinomas with Cancer Stem Cell Features
https://aacrjournals.org/cancerres/article/79/5/941/640343/miR-192-5p-Silencing-by-Genetic-Aberrations-Is-a
Construction of induced pluripotent stem cell line (ZZUi0017-A) from the fibroblast cells of a female patient with CACNA1A mutation by unintegrated reprogramming approach
https://www.sciencedirect.com/science/article/pii/S1873506120302476
FM501
Oridonin attenuates lung inflammation and fibrosis in silicosis via covalent targeting iNOS (Selected Citations)
https://doi.org/10.1016/j.biopha.2022.113532
MiR-103a-3p Promotes Zika Virus Replication by Targeting OTU Deubiquitinase 4 to Activate p38 Mitogen-Activated Protein Kinase Signaling Pathway (Selected Citations)
https://www.frontiersin.org/articles/10.3389/fmicb.2022.862580/full
Terms&Conditions:
1. Promotion is only valid from 30th April, 2023 to 30th May, 2023.
2. If the distributor's client would like to participate in the event, please contact the local distributor.
3. TransGen Biotech reserves the right to amend privileges, terms and conditions without prior notice.
4. Promotion is valid for international customers and Taiwan Province of China
Reference:
Lesley Young, Julia Sung, Glyn Stacey & John R Masters. Detection of Mycoplasma in cell cultures. Nature Protocols volume 5, pages929–934 (2010)
