Cytochrome C is an electron transfer protein located in the mitochondrial membrane space and is an essential component of the cell's electron transfer chain. When the cell is stimulated by the apoptosis signal, Cytochrome C is released from the mitochondria to the cytoplasm, accompanied by a series of changes in membrane potential, osmotic pressure and ultrastructure, promoting the activation of apoptotic caspases required for cell apoptosis.
Species reactivity
Human, mouse, rats, guinea pigs, hamsters and chlorocebus sabaeus(Species reactivity is based on WB experiment)
Isotype: Mouse IgG2a
Immunogen
• Recombinant human Cytochrome C full-length protein
• Entrez Gene ID: 54205
• UniProt ID: P99999
Applications and Suggested Dilution
• Western Blot: 1:1000-5000 dilution.
• IP: 1:100 dilution.
• IF: 1:100-200 dilution.
• FC: 1:100 dilution.
• IHC: 1:100-500 dilution(use Tris-EDTA for retrieval).
Storage
PBS (pH 7.4), 0.02% Sodium Azide, 50% Glycerol; at -20℃ for two years, avoid repeated freezing and thawing.
Shipping
Dry ice (-70 ℃)
[1]. Li K, Li Y, Shelton JM, Richardson JA, Spencer E, Chen ZJ, et al. Cytochrome c Deficiency Causes Embryonic Lethalityand Attenuates Stress-Induced Apoptosis[J]. Cell. 2000, 101 (4): 389-99.
[2]. Heiskanen KM, Bhat MB, Wang HW, Ma, Nieminen AL. Mitochondrial depolarization accompanies cytochrome c release during apoptosis in PC6 cells[J]. Journal of Biological Chemistry. 1999, 274 (9): 5654-8
[3]. Liu X, Kim CN, Yang J, Jemmerson R, Wang X. Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c[J]. Cell. 1996, 86 (1): 147-57.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
