Mesenchymal Stromal Cells (MSCs) is a kind of adult stem cell with multiple potential differentiation ability that exists in various tissues (such as bone marrow, umbilical cord, adipose, etc.). MSC also have immunomodulatory effects, so it has broad application prospects in tissue repair and the treatment of autoimmune diseases. Human MSC Characterization Kit is designed for phenotypic analysis of hMSCs by identification of hMSC surface markers through flow cytometryand. The kit contains three fluorochrome-conjugated antibodies for the identification of hMSCs, and one fluorochrome-conjugated antibody for the identification of non-MSCs. When flow cytometry is performed, the ratio of the MSC population which expresses CD105, CD73 and CD90 should be more than 95%, the ratio of the MSC population which expresses CD45 should be less than 2%. The kit uses the multicolor flow cytometry antibody co-staining method, which can minimize the cells amount required for detection. The kit is highly specific, convenient to use, and suitable for identification of hMSCs from various sources.
Storage
at 2-8°C for one year
Shipping
ice bag (4℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
