HLA-DR is a heterodimer composed of 36 kDa α chain and 27 kDa β chain, encoded by the MHC gene complex on the short arm of chromosome 6. The two chains can appear in many different allelic morphologies and are highly polymorphic. HLA-DR is structurally expressed in antigen-presenting cells, mainly in immune system cells[1]. HLA-DR was strongly expressed in monocytes, especially in CD14+ CD16+ subgroups[2]. It can also be expressed in macrophages, granulocytes, plasmoid dendritic cells, microglia and Langerhans' cells[1,3,4]. HLA-DR can also be expressed in the immature hematopoietic cells, such as BFU-E, but not in CFU-E or proerythroblast[5]. This product is the mouse anti-human HLA-DR monoclonal antibody, which is used for the specific detection of human HLA-DR by IF and FC.
Storage
PBS (pH7.4), 150 mM NaCl, 0.05% ProClin 300, 50% Glycerol; at -20℃ for two years, avoid repeated freeze-thawing.
Applicable Experiments and Dilution
· IF: 1:100 dilution is recommended.
· FC: 1:400-1:1600 dilution is recommended.
Shipping
Dry ice (-70℃)
[1] Ting JP, TROWSDALE J. Genetic control of MHC class II expression [J]. Cell. 2002, 109 Suppl: S21-33.
[2] Passlick B, Flieger D, Ziegler-Heitbrock HW. Identification and characterization of a novel monocyte subpopulation in human peripheral blood [J]. Blood. 1989, 74(7): 2527-34.
[3] Mattiace LA, Davies P, Dickson DW. Detection of HLA-DR on microglia in the human brain is a function of both clinical and technical factors [J]. Am J Pathol. 1990, 136(5): 1101-14.
[4] Angel CE, George E, Ostrovsky LL, et al. Comprehensive analysis of MHC-II expression in healthy human skin [J]. Immunol Cell Biol. 2007, 85(5): 363-9.
[5] Nakahata T, Okumura N. Cell surface antigen expression in human erythroid progenitors:erythroid and megakaryocytic markers [J]. Leuk Lymphoma. 1994, 13(5-6): 401-9.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
