CD166, also known as Activated Leukocyte Cell Adhesion Molecule (ALCAM), is a member of the immunoglobulin superfamily and has five immunoglobulin like domains (VVC2C2C2) in the extracellular region[1]. It is expressed in umbilical cord blood hematopoietic stem cells, lymphatic endothelial cells, skin, tonsil, peripheral blood mononuclear cells, and mononuclear derived dendritic cells[2-5]. In addition, CD166 promotes T cells activation and proliferation through interaction with T cell surface glycoprotein CD6[4,5]. This product is the mouse anti-human CD166 monoclonal antibody, which is used for the specific detection of human CD166 by FC.
Storage
PBS (pH7.4), 0.05% ProClin 300, 50% Glycerol; at -20℃ for two years, avoid repeated freeze-thawing.
Applicable Experiments and Dilution
· FC: 1:100 dilution is recommended.
Shipping
Dry ice (-70℃)
[1] Bowen MA, Patel DD, Li X, et al. Cloning, Mapping, and Characterization of Activated Leukocyte-Cell Adhesion Molecule (ALCAM), a CD6 Ligand [J]. J Exp Med. 1995, 181: 2213-20.
[2] Chitteti B R, Kobayashi M, Cheng Y, et al. CD166 regulates human and murine hematopoietic stem cells and the hematopoietic niche [J]. Blood. 2014, 124(4): 519-29.
[3] Iolyeva M, Karaman S, Willrodt AH, et al. Novel role for ALCAM in lymphatic network formation and function [J]. FASEB J. 2013, 27(3): 978-90.
[4] Hassan NJ, Barclay AN, Brown MH. Frontline: Optimal T cell activation requires the engagement of CD6 and CD166 [J]. Eur J Immunol. 2004, 34(4): 930-40.
[5] Zimmerman AW, Joosten B, Torensma R, et al. Long-term engagement of CD6 and ALCAM is essential for T-cell proliferation induced by dendritic cells [J]. Blood. 2006, 107(8): 3212-20.
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
