ProteinFind^® Anti-CD73 Mouse Monoclonal Antibody

CD73, also known as Ecto-5’-Nucleotidase (NT5E), is a transmembrane glycoprotein that hydrolyzes extracellular nucleotides into membrane-permeable nucleosides by anchorizing the cell membrane with Glycosylphosphatidylinositol (GPI)^[1]. CD73 is expressed in lymphocytes, mesenchymal stem cells, follicle dendritic cells, endothelial cells and epithelial cells subgroups, which synergistically stimulate T cells activation and regulate the adhesion of lymphocytes to endothelial cells^[2-5]. CD73 is used as a marker of lymphocyte differentiation, and as a result, CD73 deficiency can be found in various immune deficiency diseases^{[4, 6]}. This product is the mouse anti-human CD73 monoclonal antibody, which is used for the specific detection of human CD73 by WB, IP, IF and FC.

Applicable Experiments and Dilution

· WB: 1:1000 dilution is recommended.

· IP: 1:100 dilution is recommended.

· IF: 1:100 dilution is recommended.

· FC: 1:100 dilution is recommended.

Storage

 PBS (pH7.4), 0.05% ProClin 300, 50% Glycerol; at -20℃ for two years, avoid repeated freeze-thawing.

Shipping

Dry ice (-70℃)

[1] Le Hir M, Kaissling B. Distribution and regulation of renal ecto-5'-nucleotidase: implications for physiological functions of adenosine [J]. Am J Physiol. 1993, 264(3 Pt 2): F377-87.

[2] Minor M, Alcedo KP, Battaglia RA, et al. Cell type- and tissue-specific functions of ecto-5'-nucleotidase (CD73) [J]. Am J Physiol Cell Physiol. 2019, 317(6): C1079-C1092.

[3] Tan K, Zhu H, Zhang J, et al. CD73 Expression on Mesenchymal Stem Cells Dictates the Reparative Properties via Its Anti-Inflammatory Activity [J]. Stem Cells Int. 2019: 8717694. 

[4] Airas L, Jalkanen S. CD73 mediates adhesion of B cells to follicular dendritic cells [J]. Blood. 1996, 88(5): 1755-64.

[5] Airas L, Hellman J, Salmi M, et al. CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73 [J]. J Exp Med. 1995, 182(5): 1603-8. 

[6] Kim E-S, Ackermann C, Tóth I, et al. Down-regulation of CD73 on B cells of patients with viremic HIV correlates with B cell activation and disease progression [J]. Journal of Leukocyte Biology. 2017, 101(5): 1263-71.