Product Details
TransScript® II Multiplex Probe One-Step qRT-PCR SuperMix UDG is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube. After special optimization, this kit enables consistently amplifying multiple targets in a single reaction ensuring multiplex one-step RT-qPCR. dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent carry-over contamination.
Applications
· Singleplex to 4-plex RT-qPCR detection.
· High-copy and low-copy gene detection.
· RNA templates with high GC content or complex secondary structure.
· Detection of RNA virus or trace amounts of RNA.
Storage
at -18℃ or below for two years
Shipping
Dry ice (-70 ℃)
High sensitivity, high amplification efficiency
Using a gradient dilution (5×106 copies/mL - 5×102 copies/mL, 10-fold dilution) of the SARS-CoV-2 in vitro transcribed RNA standard and a constant concentration of Hela cell total RNA mixture as templates, amplify ORF1a, N, and RNase P genes (reference gene) with TransGen products. The results indicate that TransGen products have high amplification efficiency and sensitivity.
