TransScript® Uni Reverse Transcriptase is a reverse transcriptase with high thermal stability expressed and purified from E. coli after genetic modification and screening. It enables a broard range of reaction temperature (42°C-65°C) with ultra-high thermal stability and deficient RNase H activity. Its high reaction temperature is beneficial to open RNA secondary structure when synthesizing the first-strand cDNA, with the optimal reaction temperature of 50°C. It also has the characteristics of high sensitivity, high specificity, high yield (more full-length cDNA), High thermal stabilityand long half-life.
Features
· Ultra-high thermal stability: reaction temperature 42°C-65°C.
· High sensitivity, high specificity, high highyield and high processivity.
· The unique design of Anchored Oligo (dT)20 can anchor the first base next to the mRNA Poly (A)+5’end, and the binding site is anchored, with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis.
· Deficient RNase H activity. The degradation of the template RNA in the DNA/RNA hybrid in the first-strand cDNA synthesis reaction is avoided, thereby ensuring the yieldand length of the first-strand cDNA.
· Synthesis length can be up to 20kb.
Applications
· High-copy number and low-copy number gene detection.
· High GC or RNA template with complex secondary structure.
· cDNA library construction, primer extension, 3’ and 5’ RACE.
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
