RT-PCR
  • AU311-02.JPG
AU311-02.JPG

TransScript® -Uni One-Step gDNA Removal and cDNA Synthesis SuperMix


Product Details

TransScript®-Uni RT is an improved version of M-MLV reverse transcriptase with broad range of reaction temperature(42°C-65°C) and higher thermostability. The suggested reaction temperature is 50°C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds.

• Broad range reaction temperature (42°C -65°C ) .
• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 20 kb.

Applications
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
• cDNA library construction, primer extension, 3’ and 5’ RACE

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAU311-02 (50 rxns)AU311-03 (100 rxns)
TransScript® -Uni RT/RI Enzyme Mix50 μl100 μl
gDNA Remover50 μl100 μl
2×TS-Uni Reaction Mix500 μl1 ml
Random Primer(N9) (0.1 μg/μl)50 μl100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl)50 μl100 μl
RNase-free Water500 μl1 ml


Citations

Wu Y , Cui X , Wu N , et al. A unique case of human Zika virus infection in association with severe liver injury and coagulation disorders[J]. Scientific Reports, 2017, 7(1):11393.

Li X , Xu P , Yang X , et al. The genome sequence of a novel RNA virus inAdelphocoris suturalis[J]. Archives of Virology, 2017, 162(5):1397-1401.

Lin J , Wu Y , Han B , et al. iTRAQ-based proteomic profiling of granulosa cells from lamb and ewe after superstimulation[J]. Theriogenology, 2017:S0093691X17302893.

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