The kit provides all the necessary reagents for reverse transcription reaction (including TransScript® Uni RT, RNase Inhibitor, Anchored Oligo (dT)20 Primer, Random Primer (N9), dNTPs, Buffer). It is provided at 5× concentration and used by only adding gDNA remover, RNA template and H2O at 42°C-65°C for efficient first-strand cDNA synthesis. Simultaneously, residual genomic DNA from RNA template can be removed. 5×TransScript® Uni All-in-One No-RT Control SuperMix for qPCR is supplied to prepare no-reverse transcriptase (RT) control, which is used to assess if the qPCR template is contaminated with genomic DNA. This product is capable of minimizing contamination during operation with a simple workflow. The resulting cDNA is only suitable for qPCR, not for regular PCR.
Highlights
• Board range reaction temperature (42°C-65°C).
• "All-in-One SuperMix" form: Simultaneous cDNA synthesis and genomic DNA removal by only adding gDNA remover, RNA template and H2O
• High synthesis efficiency enabled by optimal ratio of Oligo(dT)20 Primer to Random Primer (N9) and optimized composition of the SuperMix, ensuring same reverse transcription efficiency for RNA templates of different concentrations and specifically high synthesis efficiency for short cDNA.
• Only 5 minutes for reverse transcription.
• High compatibility with qPCR reagents.
Applications
High-copy number and low-copy number gene detection
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
