RT-PCR
  • AT101-02.JPG
AT101-02.JPG

TransScript® Reverse Transcriptase


Product Details

TransScript® Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 12 kb.

Applications
• First-strand cDNA synthesis
• Multiple copy and low copy gene detection

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAT101-02AT101-03
TransScript® RT10000 U5×10000 U
5×TS RT Buffer200 μl5×200 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl)50 μl250 μl


Citations

Wu L, Liu D, Wu J, et al. Regulation of FLOWERING LOCUS T by a microRNA in Brachypodium distachyon.Plant Cell[J]. 2013, 25(11):4363-77.

Meng X , Jiang Q , Chang N , et al. Small activating RNA binds to the genomic target site in a seed-region-dependent manner[J]. Nucleic Acids Research, 2016, 44(5):2274-2282.

Xiao G , Wan Z , Fan Q , et al. The metal transporter ZIP13 supplies iron into the secretory pathway in Drosophila melanogaster.[J]. Elife, 2014, 3(16):e03191.

Tang X , Zhou B . Ferritin is the key to dietary iron absorption and tissue iron detoxification in Drosophila melanogaster[J]. The FASEB Journal, 2013, 27(1):288-298. 

Zheng J , Zhang L , Zhang J , et al. Single modification at position 14 of siRNA strand abolishes its gene-silencing activity by decreasing both RISC loading and target degradation[J]. The FASEB Journal, 2013, 27(10):4017-4026.

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