Product Details
TransScript® miRNA First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from miRNA template. High efficient poly(A) tail addition and first-strand cDNA synthesis are performed by TransScript® miRNA RT Enzyme Mix (containing tailing enzyme and RT enzyme) and 2×TS miRNA Reaction Mix. This product enables the reverse transcription of miRNA from samples such as total RNA or small RNA, and converts other RNA types present, including mRNA and snRNA, into cDNA.
• Optimized enzyme and buffer system for high efficient cDNA synthesis.
• One-step Poly(A) tailing and cDNA synthesis.
miRNA synthesis
at -18℃ or below for two years
Dry ice (-70 ℃)
| Component | AT351-01 (20 rxns) | AT351-03 (50 rxns) |
| TransScript® miRNA RT Enzyme Mix | 20 μl | 50 μl |
| 2×TS miRNA Reaction Mix | 200 μl | 500 μl |
| Universal miRNA qPCR Primer (10 μM) | 200 μl | 500 μl |
| RNase-free Water | 1 ml | 2×1 ml |
1 Zhao J, Zeng X, Liu J, et al. Marasmius androsaceus mitigates depression-exacerbated intestinal radiation injuries through reprogramming hippocampal miRNA expression[J]. Biomedicine & Pharmacotherapy, 2023.(IF 7.50)
2 You L Y, Lin J, Xu H W, et al. Intragenic heterochromatin‐mediated alternative polyadenylation modulates miRNA and pollen development in rice[J]. New Phytologist, 2021.(IF 10.15)
3 He X, Yang L, Huang R, et al. Activation of CB2R with AM1241 ameliorates neurodegeneration via the Xist/miR‐133b‐3p/Pitx3 axis[J]. Journal of cellular physiology, 2020.(IF 5.54)
