TransScript® IV Reverse Transcriptase is a modified M-MLV reverse transcriptase. The product uses RNA as a template to synthesize the first-strand cDNA, meanwhile, remove the remaining genomic DNA in the RNA template in the same reaction volume. After the reaction, it only needs to heat at 85°C for 5 seconds to inactivate TransScript® IV RT/RI Enzyme Mix and gDNA Remover at the same time. The optimal reaction temperature of the enzyme is 50 °C, and it has ultra thermal stability
Applications
·High-copy number and low-copy number gene detection.
·High GC or complex secondary structure RNA template.
·For difficult-to-handle RNA sample: degraded RNA template and RNA template containing RT enzyme inhibitors.
·cDNA library construction, primer extension, 3’ and 5’ RACE.
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
