TransScript® II Reverse Transcriptase[M-MLV,RNaseH-]（High Temperature RT）
Catalog Number： AH101-02
Price：Please inquire first
Product Details
TransScript® II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability. The enzyme is active at up to 55℃. It provides higher specificity, higher yield and more full-length cDNA products.
• Increased thermostability for more full-length cDNA products.
• Reaction temperature at 42℃-55℃.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
• cDNA up to 15 kb.
Applications
• First-strand cDNA synthesis, cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Component | AH101-02 |
| TransScript® II RT | 10000 U |
| 10×TS II RT Buffer | 100 μl |
| Anchored Oligo(dT)20 Primer (0.5 μg/μl) | 50 μl |
1 Zhang H, Yu F, Xie P, et al. A Gγ protein regulates alkaline sensitivity in crops[J]. Science, 2023.(IF 63.71)
