RT-PCR
  • AH101-02.JPG
AH101-02.JPG

TransScript® II Reverse Transcriptase


Product Details

TransScript® II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability. The enzyme is active at up to 55℃. It provides higher specificity, higher yield and more full-length cDNA products.

• Increased thermostability for more full-length cDNA products.
• Reaction temperature at 42℃-55℃.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
• cDNA up to 15 kb.

Applications
• First-strand cDNA synthesis, cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAH101-02
TransScript® II RT10000 U
10×TS II RT Buffer100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl)50 μl


Citations

Lv S , Jiang P , Nie L , et al. H+‐pyrophosphatase from Salicornia europaea confers tolerance to simultaneously occurring salt stress and nitrogen deficiency in Arabidopsis and wheat[J]. Plant Cell & Environment, 2015, 38(11):2433-2449.

Zhang F , Wang L , Li Y , et al. Optimizing mesoderm progenitor selection and three-dimensional microniche culture allows highly efficient endothelial differentiation and ischemic tissue repair from human pluripotent stem cells[J]. Stem Cell Research & Therapy, 2017, 8(1).

Md-Mustafa N , Khalid N , Gao H , et al. Transcriptome profiling shows gene regulation patterns in a flavonoid pathway in response to exogenous phenylalanine in Boesenbergia rotunda cell culture[J]. BMC Genomics, 2014, 15(1):984.

Yan S , Zou G , Li S , et al. Seed size is dete rmined by the combinations of the genes controlling different seed characteristics in rice[J]. Tag.theoretical & Applied Genetics.theoretische Und Angewandte Genetik, 2011, 123(7):1173-1181.

Ahmad A , Dong Y , Cao X . Characterization of the PRMT Gene Family in Rice Reveals Conservation of Arginine Methylation[J]. PLoS ONE, 2011, 6(8):e22664.

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