RT-PCR
  • AH411-02.JPG
AH411-02.JPG

TransScript® II One-Step RT-PCR SuperMix (+dye)


Product Details

One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. TransScript® II RT and TransTaq® HiFi DNA Polymerase are used in the kit.

Amplification of fragment up to 8 kb.

Applications
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAH411-02 (200 rxns)
TransScript® II One-Step Enzyme Mix80 μl
2×One-Step Reaction Mix2×1 ml
RNase-free Water2×1 ml


Citations

Xiao T , Wang Y , Luo H , et al. A differential sequencing-based analysis of the C. elegans noncoding transcriptome[J]. RNA, 2012, 18(4):626-639.

Fan Z , Chen X , Chen R . Transcriptome-wide analysis of TDP-43 binding small RNAs identifies miR-NID1 (miR-8485), a novel miRNA that represses NRXN1 expression[J]. Genomics, 2014, 103(1):76-82.

Zhao F , Ma J , Li L , et al. GhNAC12, a neutral candidate gene, leads to early aging in cotton (Gossypium hirsutum L)[J]. Gene, 2015, 576(1P2):268-274.

Message