RT-PCR
  • AH311-02.JPG
AH311-02.JPG

TransScript® II One-Step gDNA Removal and cDNA Synthesis SuperMix


Product Details

Unique genomic DNA remover is combined with TransScript® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis at 42-55°C. After cDNA synthesis, gDNA remover and TransScript® II RT/RI Enzyme Mix are inactivated by heating at 85°C for 5 seconds.

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.

• After reaction, heat inactivation for RT/RI and gDNA remover simultaneously can avoid damage to RNA caused by heat inactivating DNase I used for pretreatment in traditional protocol.
• Simple and fast procedure.

• cDNA up to 15 kb.

Applications
• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAH311-02 (50 rxns)AH311-03 (100 rxns)
TransScript® II RT/RI Enzyme Mix50 μl100 μl
gDNA Remover50 μl100 μl
2×TS II Reaction Mix500 μl1 ml
Random Primer (0.1 μg/μl)50 μl100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl)50 μl100 μl
RNase-free Water500 μl1 ml


Citations

Li Y , Deng H , Miao M , et al. Tomato MBD5, a methyl CpG binding domain protein, physically interacting with UV-damaged DNA binding protein-1, functions in multiple processes[J]. New Phytologist, 2016, 210(1):208-226.

Zeng J , Ding Q , Fukuda H , et al. Fertilization Independent Endosperm genes repress NbGH3.6 and regulate the auxin level during shoot development in Nicotiana benthamiana[J]. Journal of Experimental Botany, 2016, 67(8):erw024.

Zou X , Qin Z , Zhang C , et al. Over-expression of an S-domain receptor-like kinase extracellular domain improves panicle architecture and grain yield in rice[J]. Journal of Experimental Botany, 2015, 66(22):7197-7209.

Wang F , Lin R , Feng J , et al. TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana[J]. Frontiers in Plant Science, 2015, 27(6):108.

Li Q , Wang Y , Wang F , et al. Functional conservation and diversification of APETALA1/FRUITFULL genes in Brachypodium distachyon[J]. Physiologia Plantarum, 2016, 157(4):507-518.

Message