• AH301-02.JPG

TransScript® II First-Strand cDNA Synthesis SuperMix

Product Details

TransScript® II First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript® II RT/RI Enzyme Mix and 2×TS II Reaction Mix at 42-55℃, of which the optimum temperature is 50℃.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis to ensure its synthesis length and yield.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)20 Primer is specifically designed to bind to the first base next to the 5' end of Poly(A) tail of mRNA, providing higher specificity and high efficiency for first-strand cDNA synthesis.

• cDNA up to 15 kb.

• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

at -20 ℃ for two years

Dry ice (-70 ℃)

Product Contents

TransScript® II RT/RI Enzyme Mix50 μl100 μl
2×TS II Reaction Mix500 μl1 ml
Random Primer (N9) (0.1 μg/μl)50 μl100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl)50 μl100 μl
RNase-free Water500 μl1 ml


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