RT-PCR
  • AH301-02.JPG
AH301-02.JPG

TransScript® II First-Strand cDNA Synthesis SuperMix


Product Details

TransScript® II First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript® II RT/RI Enzyme Mix and 2×TS II Reaction Mix at 42-55℃, of which the optimum temperature is 50℃.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis to ensure its synthesis length and yield.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)20 Primer is specifically designed to bind to the first base next to the 5' end of Poly(A) tail of mRNA, providing higher specificity and high efficiency for first-strand cDNA synthesis.

• cDNA up to 15 kb.

Applications
• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAH301-02AH301-03
TransScript® II RT/RI Enzyme Mix50 μl100 μl
2×TS II Reaction Mix500 μl1 ml
Random Primer (N9) (0.1 μg/μl)50 μl100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl)50 μl100 μl
RNase-free Water500 μl1 ml


Citations

Wang K , Wang X , Han C , et al. From Micro to Macro: The Hierarchical Design in a Micropatterned Scaffold for Cell Assembling and Transplantation[J]. Advanced Materials, 2017, 29(2). 

Xiao J H , Yue Z , Jia L Y , et al. Obligate mutualism within a host drives the extreme specialization of a fig wasp genome[J]. Genome Biology,14,12(2013-12-20), 2013, 14(12):R141-R141.

Wang Y , Cui J , Sun X , et al. Tunneling-nanotube development in astrocytes depends on p53 activation[J]. Cell death and differentiation, 2010, 18(4):732-742.

Yue Q , Chen L , Li Y , et al. Functional Operons in Secondary Metabolic Gene Clusters in\r, Glarea lozoyensis\r, (Fungi, Ascomycota, Leotiomycetes)[J]. mBio, 2015, 6(3):e00703-15.

Cui J , Wang Y , Dong Q , et al. Morphine Protects against Intracellular Amyloid Toxicity by Inducing Estradiol Release and Upregulation of Hsp70[J]. Journal of Neuroscience, 2011, 31(45):16227-16240.

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