RT-PCR
  • AF301-02.JPG
AF301-02.JPG

TransScript® Fly First-Strand cDNA Synthesis SuperMix


Product Details

TransScript® Fly RT is generated by site mutations. It provides high affinity to RNA template with fast extension rate. The cDNA is efficiently synthesized by TransScript® Fly RT/RI Enzyme Mix and 2×TS Fly Reaction Mix. The entire reverse transcription can be completed within 5 minutes.

• 5 minutes reverse transcription.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 12 kb.

Applications
Multiple copy and low copy gene fast detection

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAF301-02AF301-03
TransScript® Fly RT/RI Enzyme Mix50 μl100 μl
2×TS Fly Reaction Mix500 μl1 ml
Random Primer (N9) (0.1 μg/μl)50 μl100 μl
Anchored Oligo (dT)18 Primer (0.5 μg/μl)50 μl100 μl
RNase-free Water500 μl1 ml


Citations

Zu G , Yao J , Ji A , et al. Nurr1 promotes intestinal regeneration after ischemia/reperfusion injury by inhibiting the expression of p21 (Waf1/Cip1)[J]. Journal of Molecular Medicine, 2016, 95(1):1-13.

Xu J , Tian Y S , Xing X J , et al. Over-expression of AtGSTU19 provides tolerance to salt, drought and methyl viologen stresses in Arabidopsis.[J]. Physiologia Plantarum, 2016, 156(2).

Jing X , Xiao-Juan X , Yong-Sheng T , et al. Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress[J]. PLOS ONE, 2015, 10(9):e0136960.

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