RT-PCR
  • AT301-02.JPG
AT301-02.JPG

TransScript® First-Strand cDNA Synthesis SuperMix


Product Details

TransScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript RT/RI Enzyme Mix and 2xTS Reaction Mix.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 12 kb.

Applications
Multiple copy and low copy gene detection

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAT301-02AT301-03
TransScript® RT/RI Enzyme Mix50 μl100 μl
2×TS Reaction Mix500 μl1 ml
Random Primer (N9) (0.1 μg/μl)50 μl100 μl
Anchored Oligo (dT)18 Primer (0.5 μg/μl)50 μl100 μl
RNase-free Water500 μl1 ml


Citations

Li S, Tian Y, Wu K, et al. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency[J]. Cell, 2018, 560(7720):595-600.

Wang S , Wu K , Qian Q , et al. Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield[J]. Cell Research, 2017, 27(9):1142-1156.

Ye J , Ge J , Zhang X , et al. Pluripotent stem cells induced from mouse neural stem cells and small intestinal epithelial cells by small molecule compounds[J]. Cell Research, 2016, 26(1):34-45. 

Xu H, Lin Z, Li F, et al. Dimerization of elongator protein 1 is essential for Elongator complex assembly[J]. Proc Natl Acad Science U S A. 2015, 112(34):10697-702.

Wang K , Hou W D , Wang X , et al. Overcoming foreign-body reaction through nanotopography: Biocompatibility and immunoisolation properties of a nanofibrous membrane[J]. Biomaterials, 2016, 102:249-258.


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