Product Details
TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The SuperMix is provided at 5× concentration and used at 1× concentration by adding RNA and H2O. The resulting cDNA are suitable for regular PCR, not for qPCR.
Highlights
• All-in-one SuperMix: Simply add RNA template and water to start the reaction.
• Optimal ratio of Oligo(dT)18 Primer and Random Primer (N9) with an optimized SuperMix composition for high-efficiency long-chain cDNA synthesis.
• PCR ready cDNA in 30 minutes (unsuitable for qPCR).
• cDNA up to 12 kb.
• Highly compatible with PCR reagents.
at -18℃ or below for two years
Dry ice (-70 ℃)
| Component | AT321-01 (50 rxns) |
| 5×TransScript® All-in-One SuperMix for PCR | 200 μl |
| RNase-free Water | 1 ml |
1 Deng P, Wang Z, Chen J, et al. RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer[J]. The Journal of Clinical Investigation, 2022.(IF 19.45)
2 Wu Z, Liu D, Pan D, et al. Structure and engineering of miniature Acidibacillus sulfuroxidans Cas12f1[J]. Nature Catalysis, 2023.(IF 37.80)
3 Liu S, Zou Q, Chen J P, et al. Targeting enhancer reprogramming to mitigate MEK inhibitor resistance in preclinical models of advanced ovarian cancer[J]. The Journal of Clinical Investigation, 2021.(IF 19.45)
4 Wang D, Zhang Y, Xu X, et al. YAP promotes the activation of NLRP3 inflammasome via blocking K27-linked polyubiquitination of NLRP3[J]. Nature communications, 2021.(IF 12.12)
5 Liu L, Liu S, Deng P, et al. Targeting the IRAK1–S100A9 Axis Overcomes Resistance to Paclitaxel in Nasopharyngeal Carcinoma[J]. Cancer Research, 2021.(IF 12.70)
