RT-PCR
  • AT321-01.JPG
AT321-01.JPG

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR


Product Details

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The SuperMix is provided at 5× concentration and used at 1× concentration by adding RNA and H2O. The resulting cDNA are suitable for regular PCR, not for qPCR.

• One-tube format for simple and fast setup and reduce pipetting variability.
• The optimal ratio of oligo(dT)18 primer to random primer(N9) for PCR-ready cDNA.
• PCR-ready cDNA in 30 minutes (unsuitable for qPCR).
• cDNA up to 12 kb.

Applications
Multiple copy and low copy gene detection

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAT321-01 (50 rxns)
TransScript® All-in-One SuperMix for PCR200 μl
RNase-free Water1 ml


Citations

Shi-Cong T , Ting Y , Yue-Lei Z , et al. Exosomes derived from miR-140-5p-overexpressing human synovial mesenchymal stem cells enhance cartilage tissue regeneration and prevent osteoarthritis of the knee in a rat model[J]. Theranostics, 2017, 7(1):180-195.

Jiansong C , Borong L , Qiuling H , et al. A novel Meloidogyne graminicola effector, MgGPP, is secreted into host cells and undergoes glycosylation in concert with proteolysis to suppress plant defenses and promote parasitism[J]. PLOS Pathogens, 2017, 13(4):e1006301.

Zhao Y F , Lai Y C , Hui G E , et al. Non-junctional Cx32 mediates anti-apoptotic and pro-tumor effects via epidermal growth factor receptor in human cervical cancer cells[J]. CELL DEATH & DISEASE, 2017, 8(5):e2773. 

Zhou W , Yao J , Wang G , et al. PKCζ phosphorylates TRAF2 to protect against intestinal ischemia–reperfusion–induced injury[J]. Cell Death & Disease, 2017, 8(7):e2935.

Hongyan W , Xiaoli T , Honglei W , et al. Proline accumulation and metabolism-related genes expression profiles in Kosteletzkya virginica seedlings under salt stress[J]. Frontiers in Plant Science, 2015, 29(6):792.

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