RT-PCR
  • AE101-02.JPG
AE101-02.JPG

EasyScript® Reverse Transcriptase


Product Details

EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity. The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 8 kb.

Applications
• First-strand cDNA synthesis
• Multiple copy gene detection

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAE101-02AE101-03
EasyScript® RT10000 U5×10000 U
5×ES RT Buffer200 μl5×200 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl)50 μl250 μl


Citations

Li K , Wang F , Cao W B . TRIB3 Promotes APL Progression through Stabilization of the Oncoprotein PML-RARα and Inhibition of p53-Mediated Senescence[J]. Cancer Cell, 2017, 31(5):697-710.

Shu J , Zhang K , Zhang M , et al. GATA family members as inducers for cellular reprogramming to pluripotency[J]. Cell Research, 2015, 25(2):169-180.

Li Y , Zhang Q , Yin X , et al. Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules[J]. CELL RESEARCH, 2010, 21(1):196-204.

Zhao Y F , Lai Y C , Hui G E , et al. Non-junctional Cx32 mediates anti-apoptotic and pro-tumor effects via epidermal growth factor receptor in human cervical cancer cells[J]. Cell Death Disease, 2017,8(5):e2773.

Ma N , Ma X , Li A , et al. Cloning and Expression Analysis of Wheat PheophorbideaOxygenase GeneTaPaO[J]. Plant Molecular Biology Reporter, 2012, 30(5):1237-1245.

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