RT-PCR
  • AE411-02.JPG
AE411-02.JPG

EasyScript® One-Step RT-PCR SuperMix (+dye)


Product Details

One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. EasyScript® RT and TransTaq®HiFi DNA Polymerase are used in the kit.

Amplification of fragment up to 4 kb.

Applications
Multiple copy gene detection

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAE411-02 (200 rxns)
EasyScript® One-Step Enzyme Mix80 μl
2×ES One-Step Reaction Mix2×1 ml
RNase-free Water2×1 ml


Citations

Dongfang H , Lin L , Jinyuan G, et al. Genetic Diversity and Positive Selection Analysis of Classical Swine Fever Virus Envelope Protein Gene E2 in East China under C-Strain Vaccination[J]. Frontiers in Microbiology, 2016,  5(7):85.

Ma X , Gu L , Li H , et al. Hypoxia-induced overexpression of stanniocalcin-1 is associated with the metastasis of early stage clear cell renal cell carcinoma[J]. Journal of Translational Medicine, 2015, 13(1):56.

Rotimi OA, Rotimi SO, Duru CU, et al.    Acute aflatoxin B1 – Induced hepatotoxicity alters gene expression and disrupts lipid and lipoprotein metabolism in rats[J]. Toxicol Report. 2017,24(4): 408-414. 

Mao L , Li W , Liu X , et al. Chinese border disease virus strain JSLS12-01 infects piglets and down-regulates the antibody responses of classical swine fever virus C strain vaccination[J]. Vaccine, 2015, 33(32):3918-3922.

Liu Z Z , Xie X D , Qu S X , et al. Small breast epithelial mucin (SBEM) has the potential to be a marker for predicting hematogenous micrometastasis and response to neoadjuvant chemotherapy in breast cancer[J]. Clinical & Experimental Metastasis, 2010, 27(4):251-259.

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