RT-PCR
  • AE311-02.JPG
AE311-02.JPG

EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix


Product Details

Unique genomic DNA remover is combined with EasyScript® First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 8 kb.

Applications
Multiple copy gene detection

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAE311-02 (50 rxns)AE311-03 (100 rxns)AE311-04 (500 rxns)
EasyScript® RT/RI Enzyme Mix50 μl100 μl5×100 μl
gDNA Remover50 μl100 μl5×100 μl
2×ES Reaction Mix500 μl1 ml5×1 ml
Random Primer(N9) (0.1 μg/μl)50 μl100 μl5×100 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl)50 μl100 μl5×100 μl
RNase-free Water500 μl1 ml5 ml


Citations

Li S, Tian Y, Wu K, et al. Modulating plant growth–metabolism coordination for sustainable agriculture[J]. Nature, 2018, 560(7720):595-600.

Li P, Du J, Goodier JL, et al. Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion[J]. Nucleic Acids Research. 2017, 45(8):4619-4631. 

Ju Z , Cao D , Gao C, et al. A Viral Satellite DNA Vector (TYLCCNV) for Functional Analysis of miRNAs and siRNAs in Plants[J]. Plant Physiology, 2017, 173(4):1940-1952.

Tongjin L , Xiaohui Z , Haohui Y, et al. Aromatic Glucosinolate Biosynthesis Pathway in Barbarea vulgaris and its Response to Plutella xylostella Infestation[J]. Frontiers in Plant Science, 2016, 7.

5.Sun W , Shen Y H , Zhou L X , et al. Ecd

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