RT-PCR
  • AE301-02.JPG
AE301-02.JPG

EasyScript® First-Strand cDNA Synthesis SuperMix


Product Details

EasyScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by EasyScript® RT/RI Enzyme Mix and 2×ES Reaction Mix.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the, product obtained from 30 minutes reaction is used for PCR.
Anchored Oligo(dT)18 Primer is specifically designed to bind to the first base next to mRNA Poly(A)+ on the 5' end with high specificity, ensuring high efficiency and success rate of first-strand cDNA synthesis.
• Random Primer (N9) or Gene Specific Primer (GSP) can be used to synthesize the first-strand cDNA.

• cDNA up to 8 kb.

Applications
Multiple copy gene detection

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentAE301-02AE301-03
EasyScript® RT/RI Enzyme Mix50 μl100 μl
2×ES Reaction Mix500 μl1 ml
Random Primer(N9) (0.1 μg/μl)50 μl100 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl)50 μl100 μl
RNase-free Water500 μl1 ml


Citations

Li P, Du J, Goodier JL, et al. Aicardi–Gouti`eres syndrome protein TREX1 suppresses L1 and maintains genome integrity through exonuclease-independent ORF1p depletion[J]. Nucleic Acids Resersch. 2017, 45(8):4619-4631.

Chen L , Li J , Wang F , et al. Tie2 expression on macrophages is required for blood vessel reconstruction and tumor relapse after chemotherapy[J]. Cancer Research, 2016:0008-5472.CAN-16-1114.

Zhu F F , Zhang P B , Zhang D H , et al. Generation of pancreatic insulin-producing cells from rhesus monkey induced pluripotent stem cells[J]. Diabetologia, 2011, 54(9):2325-2336.

Geng C, Cong Q Q, Li X D, et al. DEVELOPMENTALLY REGULATED PLASMA MEMBRANE PROTEIN of Nicotiana benthamiana contributes to potyvirus movement and transports to plasmodesmata via the early secretory pathway and the actomyosin system[J]. Plant Physiology, 2015, 167(2):394-410.

Feng X , Xu X , Xiao X , et al. NMI inhibits cancer stem cell traits by downregulating hTERT in breast cancer[J]. Cell Death & Disease, 2017, 8(5):e2783.

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