Product Details
This product contains all the necessary reagents for the reverse transcription reaction, including EasyScript® RT, RNase Inhibitor, Anchored Oligo(dT)₁₈ Primer, Random Primer (N9), dNTPs, and Buffer, at a optimized 5× concentration. To efficiently synthesize first-strand cDNA while simultaneously removing residual genomic DNA from the RNA template, simply add the gDNA Remover, template RNA, and water. The kit also includes a 5× EasyScript® All-in-One No-RT Control SuperMix for qPCR for setting up a no-reverse-transcriptase control reaction. This control is essential for determining whether the qPCR signal originates from the cDNA or from contaminating genomic DNA. The pre-mixed formulation offers a simple and convenient protocol, significantly reducing the risk of contamination during setup. The synthesized cDNA is specifically intended for use in qPCR only, and is not recommended for regular PCR.
Features
• “All-in-One SuperMix” type: Simultaneous cDNA synthesis and genomic DNA removal by only adding gDNA remover, RNA template and H2O
• High synthesis efficiency enabled by optimal ratio of Oligo(dT)18 Primer to Random Primer (N9) and optimized composition of the SuperMix, ensuring same reverse transcription efficiency for RNA templates of different concentrations and specifically high synthesis efficiency for short cDNA.
• Only 15 minutes for reverse transcription.
• High compatibility with qPCR reagents.
Applications
High-copy-number and low-copy number gene detection
Storage
at -18 ℃ or below for two years
Shipping
Dry ice (-70 ℃)
| Component | AE341-02 (100 rxns) | AE341-03 (500 rxns) |
| 5×EasyScript® All-in-One SuperMix for qPCR | 400 μl | 5×400 μl |
| 5×EasyScript® All-in-One No-RT Control SuperMix for qPCR | 40 μl | 200 μl |
| gDNA Remover | 100 μl | 5×100 μl |
| Nuclease-free Water | 2×1 ml | 2×5 ml |
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