RNA Purification
  • ER501-01.JPG
ER501-01.JPG

TransZol  Up Plus RNA Kit


Product Details

TransZol Up Plus RNA Kit is suitable for the isolation of total RNA from cells and tissues. After the sample is lysed with TransZol Up and RNA Extraction Agent is added, the solution separates into an upper colorless aqueous phase (containing RNA), an interphase and a lower pink organic phase. A silica-based spin column is used to specifically bind to RNA in the aqueous phase. Compared with other total RNA extraction methods, TransZol Up Plus RNA Kit possesses not only advantages of strong lysis capability, high yield and a wide range of applications of TransZol Up, but also the advantage of high purity of spin column extraction.


Highlights

• High operational safety: RNA Extraction Agent replaces chloroform.

• A wide range of applications enabled by the maximum binding capacity of the spin column of 100 μg: animal and plant tissues, viruses, bacteria or other samples. Small amount of samples (50-100 mg tissues, 5×106cells, or 200 μl blood) and large amount of samples (≥1g tissues or ≥107cells).

• Strong lysis capability: Complete and fast lysis, high yield.

• Rapid extraction: Completed in one hour.

• Visible workflow: Pink organic phase to facilitate separation from colorless aqueous phase

• High purity: Minimum DNA and protein contamination.


Storage
TransZol Up at 2-8℃ away from light for one year, others at room temperature (15℃- 25℃) in a dry place for one year


Shipping
TransZol Up on ice bag (4°C) ; others at room temperature.

Product Contents


ComponentER501-01 (100 rxns)
TransZol Up100 ml
RNA Extraction Agent20 ml
Clean Buffer 9 (CB9)110 ml
Wash Buffer 9 (WB9)24 ml
RNase-free Water40 ml
RNase-free Tube (1.5 ml)100
RNA Spin Column with Collection Tubes100


Citations

Wei W , Liu X , Chen J , et al. Class I histone deacetylases are major histone decrotonylases: evidence for critical and broad function of histone crotonylation in transcription[J]. Cell Research.

Zenghui H , Biao T , Qi W , et al. Transcriptome Sequencing Analysis Reveals a Difference in Monoterpene Biosynthesis between Scented Lilium ‘Siberia’ and Unscented Lilium ‘Novano’[J]. Frontiers in Plant Science, 2017, 8:1351.

Xu L , Yin L , Tao X , et al. Dioscin, a potent ITGA5 inhibitor, reduces the synthesis of collagen against liver fibrosis: Insights from SILAC-based proteomics analysis[J]. Food & Chemical Toxicology An International Journal Published for the British Industrial Biological Research Association, 2017, 107(Pt A):318.


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