TransZol Up Plus RNA Kit is suitable for the isolation of total RNA from cells and tissues. After the sample is lysed with TransZol Up and chloroform is added, the solution separates into an upper colorless aqueous phase (containing RNA), an interphase and a lower pink organic phase. A silica-based spin column is used to specifically bind to RNA in the aqueous phase. Compared with other total RNA extraction methods, TransZol Up Plus RNA Kit possesses not only advantages of strong lysis capability, high yield and a wide range of applications of TransZol Up, but also the advantage of high purity of spin column extraction.
Highlights
• A wide range of applications enabled by the maximum binding capacity of the spin column of 100 μg: animal and plant tissues, viruses, bacteria or other samples. Small amount of samples (50-100 mg tissues, 5×106 cells, or 200 μl blood) and large amount
of samples (≥1g tissues or ≥107 cells).
• Strong lysis capability: Complete and fast lysis, high yield.
• Rapid extraction: Completed in one hour.
• Visible workflow: Pink organic phase to facilitate separation from colorless aqueous phase
• High purity: Minimum DNA and protein contamination.
Storage
TransZol Up at 2-8°C away from light for one year, others at room temperature (15°C- 25°C) in a dry place for one year
Shipping
ice bag (4°C)
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