Restriction Enzymes
  • 内切酶系列.JPG
内切酶系列.JPG

FlyCut® SphI


Product Details

FlyCut® SphI is expressed and purified from E.coli that carries the recombinant SphI gene. The molecular weight is 26.8 kDa, with the recognition site at GCATG^C. The reaction is conducted for 5-10 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...GCATG↓C...3’
3’...C↑GTACG...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentJS701-01JS701-02
FlyCut® SphI250 units2×250 units
10×FlyCut® Buffer250 μl250 μl
10×DNA Loading Buffer1 ml1 ml


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