Restriction Enzymes
  • 内切酶系列.JPG
内切酶系列.JPG

FlyCut® SpeI


Product Details

FlyCut® SpeI is expressed and purified from E.coli that carries the recombinant SpeI gene. The molecular weight is 21.7 kDa, with the recognition site at A^CTAGT. The reaction is conducted for 5-15 minutes at 37oC, and heat-inactivated at 80oC for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...ACTAGT...3’
3’...TGATCA...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentJS601-01JS601-02
FlyCut® SpeI250 units2×250 units
10×FlyCut® Buffer250 μl250 μl
10×DNA Loading Buffer1 ml1 ml


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