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Restriction Enzymes
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内切酶系列.JPG

FlyCut® SpeI


Product Details

FlyCut® SpeI is expressed and purified from E.coli that carries the recombinant SpeI gene. The molecular weight is 21.7 kDa, with the recognition site at A^CTAGT. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...A↓CTAGT...3’
3’...TGATC↑A...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentJS601-01JS601-02
FlyCut® SpeI250 units2×250 units
10×FlyCut® Buffer250 μl250 μl
10×DNA Loading Buffer1 ml1 ml


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