Restriction Enzymes
  • 内切酶系列.JPG
内切酶系列.JPG

FlyCut® SmaI


Product Details

FlyCut® SmaI is expressed and purified from E.coli that carries the recombinant SmaI gene. The molecular weight is 29.7 kDa, with the recognition site at CCC^GGG The reaction is conducted for 5-10 minutes at 25oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.

Recognition Site
5’...CCC↓GGG...3’
3’...GGG↑CCC...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentJS501-01JS501-02
FlyCut® SmaI1,000 units2×1,000 units
10×FlyCut® Buffer250 μl500 μl
10×DNA Loading Buffer1 ml1 ml


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