Restriction Enzymes
  • 内切酶系列.JPG
内切酶系列.JPG

FlyCut® NotI


Product Details

FlyCut® NotI is expressed and purified from E.coli that carries the recombinant NotI gene. The molecular weight is 43.3 kDa, with the recognition site at GC^GGCCGC. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Recognition Site
5’...GC↓GGCCGC...3’
3’...CGCCGG↑CG...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentJN401-01JN401-02
FlyCut® NotI250 units2×250 units
10×FlyCut® Buffer250 μl250 μl
10×DNA Loading Buffer1 ml1 ml


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