Restriction Enzymes
  • 内切酶系列.JPG
内切酶系列.JPG

FlyCut® NdeI


Product Details

FlyCut® NdeI is expressed and purified from E.coli that carries the recombinant NdeI gene. The molecular weight is 46.5 kDa, with the recognition site at CA^TATG. The reaction is conducted for 5-10 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site
5’...CA↓TATG...3’
3’...GTAT↑AC...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentJN201-01JN201-02
FlyCut® NdeI2,000 units2×2,000 units
10×FlyCut® Buffer500 μl1 ml
10×DNA Loading Buffer1 ml1 ml


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