Restriction Enzymes
  • 内切酶系列.JPG
内切酶系列.JPG

FlyCut® EcoRI


Product Details

FlyCut® EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa, with the recognition site at G^AATTC. The reaction is conducted for 5-10 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam, dcm methylation, but sensitive to mammalian CpG methylation.

Recognition Site
5’...G↓AATTC...3’
3’...CTTAA↑G...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C  for two years

Shipping
Dry ice (-70 ℃)

Product Contents


ComponentJE201-01JE201-02
FlyCut® EcoRI5,000 units2×5,000 units
10×FlyCut® Buffer2×1 ml3×1 ml
10×DNA Loading Buffer1 ml1 ml


References

1.Lu C , Ma J , Cai D . Increased HAGLR expression promotes non–small cell lung cancer proliferation and invasion via enhanced de novo lipogenesis[J]. Tumor Biology, 2017, 39(4):101042831769757.

2.Jian‑Zhi Gao, Li J , Jing‑Li Du, et al. Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence[J]. Oncology letters, 2016, 11(3):1791-1798.


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