Residual DNA Detection
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PerfectStart® Pichia DNA Quantification Kit

Product Details

This product is used for quantitative detection of Pichia DNA remaining in various biological products.  The principle of this product is that the amount of nucleic acid in the sample is determined by the detection of the intensity of  fluorescence which is proportional to the amount of amplified product during the amplification process by adding a fluorescent probe  (TaqMan or Molecular Beacon, etc.) to the PCR system. Pichia qPCR SuperMix (2×) in this product contains PerfectStart Taq hot  start enzyme (uses 3 monoclonal antibodies to efficiently bind with Taq DNA Polymerase, effectively blocking DNA polymerase  activity and preventing non-specific amplification at low temperature), qPCR reaction buffer, dNTPs, PCR enhancer and stabilizer  specially optimized for Pichia DNA detection. In addition, a dUTP/UDG system is introduced into this reaction mix, which can  degrade U-containing ssDNA and dsDNA before reverse transcription, eliminating cross-contamination caused by PCR products.


 • High specificity, high sensitivity, high amplification efficiency enabled by 3 kinds of blocking antibodies, and suitable for a wide  range of samples. 

 • Specially optimized qPCR reaction buffer to provide higher extension speed, sensitivity and specificity.  

 • The use of UDG enzyme and dUTP can effectively prevent cross-contamination of PCR products to ensure accurate data.

at -20 ℃ for two years

Dry ice (-70 ℃)

Product Contents

Pichia qPCR SuperMix (2×)2 × 750 μlPCR enzymes, dNTPs,  Mg2+, PCR buffer, etc.
6 × Pichia Primer Probe Mix500 μlPichia DNA primer probe
Pichia DNA Standard S0100 μlPichia standard DNA
Standard Diluent3 ×1 ml
Nuclease-free Water1 ml

PCR enzymes, dNTPs, Mg2+, PCR buffer, etc.

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