PerfectStart® II Probe qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except probe, primer and template. It contains PerfectStartTM Taq DNA Polymerase (PerfectStart® Taq DNA polymerase is a hot-start Taq DNA polymerase containing Taq DNA polymerase and three kinds of monoclonal antibodies, effectively blocking DNA polymerase activity and preventing non-specific amplification at low temperature), specially optimized dual-cation buffer, dNTPs, PCR enhancer and stabilizer. qPCR SuperMix is provided at 2×concentration and can be used at 1×concentration by adding template, primer, probe, passive reference dye (optional) and Nuclease-free Water. dUTP/ UDG is included in the kit to degrade dU-containing ssDNA and dsDNA, which can prevent cross contamination.
Highlights
• PerfectStart® Taq DNA Polymerase, hot start with three antibodies blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
• Specially optimized qPCR reaction buffer provides higher sensitivity and specificity.
• dUTP/ UDG used to avoid cross contamination.
• Passive reference dyes are provided for different qPCR instruments.
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
