PerfectStart® III Fast Probe qPCR SuperMix UDG is supplied at a 2× concentration, and is a ready-to-use cocktail containing all components, except probes, primers and template for the amplification and detection of DNA in real-time qPCR. Simply add primers, probe, template, and Nuclease-free Water to perform singleplex, multiplex (up to quadruple) PCR reactions in a single well. PerfectStart® Taq III DNA Polymerase(using antibody blocking method to effectively block the activity of DNA polymerase to prevent non-specific amplification at low temperature), combined with optimal reaction buffer, which significantly improves template affinity, qPCR amplification performance and the sensitivity of low-concentration template detection. It supports full premixing of primers and probes, and has good impurity tolerance to low-purity nucleic acids (presence of inhibitors), which is suitable for a variety of detection scene. The dUTP/UDG in the SuperMix can function at room temperature to eliminate cross-contamination caused by PCR products and ensure the accuracy of results.
Applications
Detection of DNA of animal, plant and microorganism by probe fluorescence quantitative PCR
Highlights
• PerfectStart® Taq III DNA Polymerase, hot start with three antibodies blocking technique, improves sensitivity, enhances
specificity and generates more accurate data.
• Specially optimized qPCR reaction buffer provides higher sensitivity and specificity.
• High-efficiency antibody blocking, with optimal qPCR reaction buffer, supports full premixing of primers and probes, and
enhances anti-suppression.
• The dUTP/UDG anti-contamination system is used to effectively prevent cross-contamination of PCR products.
• Passive reference dyes are provided for different qPCR instruments
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
Bio-Medicine
In Vitro Diagnostics
PCR, RT-PCR, qPCR, qRT-PCR
Restriction/Modifying Enzymes
DNA Molecular Weight Standards
Cloning and Mutagenesis System
Nucleic Acid Purification
Next Generation Sequencing
Gene Expression
Protein Analysis
Protein MW Standards
Cell Culture and Detection
Exosome Related
Serum-free medium
Antibodies
ELISA
Instruments
Residual DNA Detection
