Protein Extraction
  • DE201-01.JPG
DE201-01.JPG

ProteinExt® Mammalian Nuclear and Cytoplasmic Protein Extraction Kit


Product Details

ProteinExt® Mammalian Nuclear and Cytoplasmic Protein Extraction Kit provides a fast and efficient method to extract nuclear and cytoplasmic proteins from mammalian cells and tissues. Native proteins can be obtained within 80 minutes without ultracentrifugation. The extracted proteins are suitable for a variety of downstream applications, including SDS-PAGE, Western blot, ELISA, enzyme-activity assays, immunoprecipitation and transcription factor activity analysis.

Storage
ProteinExtTM Protease Inhibitor Cocktail, EDTA-free (100×) at -20℃ for one year; Others at 2-8℃ for one year.

Shipping
ProteinExtTM Protease Inhibitor Cocktail, EDTA-free (100×) on dry ice (-70℃); Others on ice bag (4℃). 

Product Contents


ComponentDE201-01 (50 rxns)
Cytoplasmic Protein Extraction Buffer I (CPEB I)75 ml
Cytoplasmic Protein Extraction Buffer II (CPEB II)3 ml
Nuclear Protein Extraction Buffer (NPEB)25 ml
ProteinSafeTM Protease Inhibitor Cocktail, EDTA-free (100×)1 ml


Citations

Liu Y , Fang S , Li X , et al. Aspirin inhibits LPS-induced macrophage activation via the NF-κB pathway[J]. Sci Rep, 2017, 7(1):11549.

Xu X , Qimuge A , Wang H , et al. IRE1 alpha XBP1s branch of UPR links HIF1 alpha activation to mediate ANGII-dependent endothelial dysfunction under particulate matter (PM) 2.5 exposure[J]. SCIENTIFIC REPORTS, 2017, 7(1):13507.

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