Protein Extraction
  • DE401-01.JPG
DE401-01.JPG

ProteinExt® Mammalian Mitochondria Isolation Kit for Cultured Cells


Product Details

ProteinExt® Mammalian Mitochondria Isolation Kit for Cultured Cells provides a fast and efficient method to isolate mitochondria from cultured mammalian cells. This kit provides two options for the separation of mitochondria from cytosolic components: a reagent-based method or homogenization-based method. Reagent-based method uses a mild procedure to process single or multiple samples. The isolated mitochondria is suitable for a variety of downstream applications, including protein analysis, apoptosis, signal transduction and metabolic assays.

Storage
ProteinExtTM Protease Inhibitor Cocktail, EDTA-free (100×)and MSB at -20℃ for one year; Others at 2-8℃ for one year.

Shipping
ProteinExtTM Protease Inhibitor Cocktail, EDTA-free (100×) and MSB on dry ice (-70℃); Others on ice bag (4℃). 

Product Contents


ComponentDE401-01 (50 rxns)
Mitochondria Isolation Buffer I (MIB I)50 ml
Mitochondria Isolation Buffer II (MIB II)500 μl
Mitochondria Isolation Buffer III (MIB III)65 ml
Mitochondria Storage Buffer (MSB)4 ml
ProteinSafeTM Protease Inhibitor Cocktail, EDTA-free (100×)1.2 ml


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